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Production of Monoclonal Antibodies against Yellowtail Leucocyte
http://hdl.handle.net/10126/4351
http://hdl.handle.net/10126/435143cf6353-5507-44de-9dd9-2ac4dd784dbf
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
marine18-027.pdf (1.0 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-03-17 | |||||
| タイトル | ||||||
| タイトル | Production of Monoclonal Antibodies against Yellowtail Leucocyte | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Monoclonal antibody | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | yellowtail | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | leucocyte | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | classification of leucocytes | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Ishikawa, Takahide
× Ishikawa, Takahide× Kawai, Kenji |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | BALB/c mouse was immunized with yellowtail peripheral blood leucocytes (PBLs) and spleen cells removed from the immunized mouse were fused with myeloma by using polyethyleneglycol method. Antibody producing hybridomas were screened by ELISA using mixed leucocytes as target antigen. A total of 8 hybridomas (culture no. AB-2, AB-10, BB-11, BC-5, BC-6, CF-11, DG-10 and EG-11) were obtained by single cell cloning. Monoclonal antibodies (MAbs) produced in the culture supernatant of cloned hybridomas were used for further characterization. Imunofluorescent stain using FITC labeled rabbit secondary antibody revealed that MAbs AB-2, AB-10 and EG-11 recognized lymphocyte and MAbs BC-5, CF-11 and DG-10 recognized granulocyte or monocyte. MAbs BC-6 and BB-11 reacted to yellowtail lymphocyte which bore immunoglobulin of yellowtail. | |||||
| 書誌情報 |
Bulletin of marine sciences and fisheries, Kochi University 巻 18, p. 27-31, 発行日 1998-12-31 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03879763 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10778872 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版者 | ||||||
| 出版者 | 高知大学海洋生物教育研究センター | |||||
| 言語 | ja | |||||
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Cite as
Ishikawa, Takahide, Kawai, Kenji, 1998, Production of Monoclonal Antibodies against Yellowtail Leucocyte: 高知大学海洋生物教育研究センター, 27–31 p.
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