{"created":"2023-05-15T15:32:04.375086+00:00","id":2066,"links":{},"metadata":{"_buckets":{"deposit":"74df9e52-7f68-419c-bf5f-9d8c9c22343d"},"_deposit":{"created_by":3,"id":"2066","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2066"},"status":"published"},"_oai":{"id":"oai:kochi.repo.nii.ac.jp:00002066","sets":["98:117:125"]},"author_link":["10871","10872"],"control_number":"2066","item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-12-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"31","bibliographicPageStart":"27","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"Bulletin of marine sciences and fisheries, Kochi University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BALB/c mouse was immunized with yellowtail peripheral blood leucocytes (PBLs) and spleen cells removed from the immunized mouse were fused with myeloma by using polyethyleneglycol method. Antibody producing hybridomas were screened by ELISA using mixed leucocytes as target antigen. A total of 8 hybridomas (culture no. AB-2, AB-10, BB-11, BC-5, BC-6, CF-11, DG-10 and EG-11) were obtained by single cell cloning. Monoclonal antibodies (MAbs) produced in the culture supernatant of cloned hybridomas were used for further characterization. Imunofluorescent stain using FITC labeled rabbit secondary antibody revealed that MAbs AB-2, AB-10 and EG-11 recognized lymphocyte and MAbs BC-5, CF-11 and DG-10 recognized granulocyte or monocyte. MAbs BC-6 and BB-11 reacted to yellowtail lymphocyte which bore immunoglobulin of yellowtail.","subitem_description_type":"Abstract"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"高知大学海洋生物教育研究センター","subitem_publisher_language":"ja"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03879763","subitem_source_identifier_type":"ISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10778872","subitem_source_identifier_type":"NCID"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishikawa, Takahide"}],"nameIdentifiers":[{"nameIdentifier":"10871","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawai, Kenji"}],"nameIdentifiers":[{"nameIdentifier":"10872","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-23"}],"displaytype":"detail","filename":"marine18-027.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"marine18-027.pdf","url":"https://kochi.repo.nii.ac.jp/record/2066/files/marine18-027.pdf"},"version_id":"16d1cf35-78b1-4fc7-9a0c-b5648d7a9877"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Monoclonal antibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"yellowtail","subitem_subject_language":"en"},{"subitem_subject":"leucocyte","subitem_subject_language":"en"},{"subitem_subject":"classification of leucocytes","subitem_subject_language":"en"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Production of Monoclonal Antibodies against Yellowtail Leucocyte","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Production of Monoclonal Antibodies against Yellowtail Leucocyte","subitem_title_language":"en"}]},"item_type_id":"4","owner":"3","path":["125"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-03-17"},"publish_date":"2010-03-17","publish_status":"0","recid":"2066","relation_version_is_last":true,"title":["Production of Monoclonal Antibodies against Yellowtail Leucocyte"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-19T01:08:25.675342+00:00"}