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ブリ連鎖球菌症に対する経口ワクチンのマイクロカプセル化
http://hdl.handle.net/10126/4366
http://hdl.handle.net/10126/43663dd4d7a1-f2a1-485b-be6d-b4279b02ca97
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
marine19-071.pdf (923.9 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-03-17 | |||||
| タイトル | ||||||
| タイトル | ブリ連鎖球菌症に対する経口ワクチンのマイクロカプセル化 | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Encapsulation of oral delivery vaccine against Lactococcus garvieae infection in yellowtail Seriola quinqueradiata | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Microcapsule | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | oral vaccine | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Lactococcus garvieae | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | yellowtail | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
川合, 研児
× 川合, 研児× 畑本, 琴美 |
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| 著者別名 | ||||||
| 姓名 | Kawai, Kenji | |||||
| 著者別名 | ||||||
| 姓名 | Hatamoto, Kotomi | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Oral vaccine against streptococcus infection in yellowtail Seriola quinqueradiata is already commercialized, however the vaccine has disadvantageous property that much amount is necessary to induce enough efficacy. One of the proposed reasons for it is that antigenicity of the administrated antigen is decreased while it passes the stomach of fish. Present study is conducted to develop encapsulated vaccine to protect antigen from the effect of stomach. Firstly, culture and inactivation conditions of the bacterium Lactococcus garvieae were examined to get highly antigenic cells. L. garvieae S−2433 strain was cultured with and without shaking in brain heart infusion broth. Antigenicity was determined by agglutination using antisera against two types of L. garvieae. Antigenicity was also determined after inactivation with various concentrations of formalin and by heating at different temperatures. Encapsulation of vaccine was carried out by the method of mixing acetone dissolution of cellulose acetate phthalate to antigen dispersed in paraffin oil. Confirmation of encapsulation of the antigen was carried out using Ouchterlony immunodiffusion test. The highest agglutination of the bacterium was found when it was cultured 9-12 h with shaking at 30℃. High antigenicity of the bacterium is shown when it was inactivated with 0.2 % formalin or by heating for 30 min at 100℃. The diameter of the capsule preparation ranged 30-70 μm, showing spherical body. The result of Ouchterlony test showed that the antigen was encapsulated and the encapsulated antigen was released at neutral pH but not at low pH. Thus, a possibility that this enteric-coated microcapsule is available for the development of new oral vaccine. | |||||
| 書誌情報 |
Bulletin of marine sciences and fisheries, Kochi University 巻 19, p. 71-78, 発行日 2000-03-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03879763 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10778872 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版者 | ||||||
| 出版者 | 高知大学海洋生物教育研究センター | |||||
| 言語 | ja | |||||
