| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-03-03 |
| タイトル |
|
|
タイトル |
Evolution and Functional Diversification of Serine Racemase Homologs in Bacteria |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
D-amino acid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Aspartate racemase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Serine racemase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Arginine racemase |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Kouji, Uda
Rie, Nishimura
Yuexuan, Li
Eisaku, Shimoda
Tetsuya, Miyamoto
Luke A. Moe
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Amino acid racemases catalyze the interconversion of l- and d-amino acids, maintaining intracellular levels of both d- and l-amino acids. While alanine and glutamate racemases are widespread in bacteria, serine racemase (SerR) is predominantly found in animals. Recently, homologs of animal SerR were reported in some bacterial genomes, but their evolutionary distribution
and functional roles remain poorly understood. In this study, we cloned and expressed 20 SerR homologous genes from 13 bacterial species spanning five phyla and characterized their enzymatic activity. Six homologs exhibited serine dehydratase activity, while the remaining showed racemase activity with serine, aspartate, asparagine, or arginine. Notably,the SerR homologs from Parafannyhessea umbonata (Actinomycetota), Clostridium aceticum, Anaerovirgula multivorans,Alkaliphilus oremlandii (Bacillota), Acetomicrobium mobile, and Thermovirga lienii (Synergistota) demonstrated strong arginine racemase activity, with Km values ranging from 0.167 to 0.885 mM and kcat values ranging from 5.86 to 61.5 s−1 for l-arginine. Phylogenetic analysis revealed that bacterial and eukaryotic SerR homologs share a common ancestral gene, and substrate specificity has independently changed multiple times during evolution. Amino acid sequence alignment and analysis of site-directed mutants revealed that residues at positions 146 to 148 and surrounding regions, located near the substrate-binding site, play a crucial role in substrate specificity and/or catalytic activity. These results highlight the evolutionary processes that drive functional diversification in serine racemase homologs. |
|
言語 |
en |
| bibliographic_information |
en : Journal of Molecular Evolution
巻 93,
号 1,
p. 149-162,
発行日 2025-02
|
| item_11_source_id_7 |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
1432-1432 |
| item_11_relation_11 |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1007/s00239-024-10231-7 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/. |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
Springer |
|
言語 |
en |
Journal of Molecalar Evolution_93_1_149.pdf (1.3 MB)
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