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Cytoskeletal and Ultrastructural Studies on Regenerating Cells from the Protoplasts of Valonia (Siphonocladales, Chlorophyceae)
http://hdl.handle.net/10126/4823
http://hdl.handle.net/10126/48232929e521-26e3-41b2-8b4a-f9dd9a71c823
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
kuro5-1.39.pdf (1.0 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-02-28 | |||||
| タイトル | ||||||
| タイトル | Cytoskeletal and Ultrastructural Studies on Regenerating Cells from the Protoplasts of Valonia (Siphonocladales, Chlorophyceae) | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | actin filament | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | cortical microtubule | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | protoplast | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Valonia | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Paul, Rommel Elvira
× Paul, Rommel Elvira× Kazuo, Okuda |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Ultrastructure and cytoskeletal properties of the coenocytic green alga, Valonia, were described using light field, immunofluorescence and electron microscopy to investigate the dynamics among cell wall, cell membrane, and protoplasm during cell regeneration. Protoplasts were artificially induced in three species by cutting thalli and extruding the protoplasm. Protoplasts contracted and formed irregularly shaped masses within 30 minutes concomitant with bundling of actin filaments (AFs), convolution of cortical microtubules (CMTs) and formation of a thin enveloping membrane composed of polysaccharides.Size affected survival rates: protoplasts less than 10 μm in diameter displayed lower viabilitythan larger protoplasts. A new cell wall was produced within 24 hours simultaneous with CMT andAF depolymerization. AFs were reduced to granular structures and aggregates that repolymerizedby 48 hours. Concurrently, new CMTs polymerized and attained a parallel arrangement. Actin- andmicrotubule-destabilizing agents had variable effects on protoplast contraction indicating a minor role of intact cytoskeletons in this process; however, resulting cells exhibited abnormal protoplasm distribution and cell deformation after three days. Rhizoids began to form after 7 days on untreated cells which subsequently produced lateral branch cells that eventually developed into mature thalli. | |||||
| 書誌情報 |
黒潮圏科学 = Kuroshio Science 巻 5, 号 1, p. 39-49, 発行日 2011-03-25 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1882823X | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12321175 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版者 | ||||||
| 出版者 | Graduate School of Kuroshio Science, Kochi University | |||||
| 言語 | en | |||||
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Cite as
Paul, Rommel Elvira, Kazuo, Okuda, 2011, Cytoskeletal and Ultrastructural Studies on Regenerating Cells from the Protoplasts of Valonia (Siphonocladales, Chlorophyceae): Graduate School of Kuroshio Science, Kochi University, 39–49 p.
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