{"created":"2023-05-15T15:35:08.863964+00:00","id":6454,"links":{},"metadata":{"_buckets":{"deposit":"37710520-e8d6-4e35-998b-0f0dd9fd6aa6"},"_deposit":{"created_by":3,"id":"6454","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"6454"},"status":"published"},"_oai":{"id":"oai:kochi.repo.nii.ac.jp:00006454","sets":["100:576:581"]},"author_link":["24414","24415"],"control_number":"6454","item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-03-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"49","bibliographicPageStart":"39","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"黒潮圏科学 = Kuroshio Science"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ultrastructure and cytoskeletal properties of the coenocytic green alga, Valonia, were described using light field, immunofluorescence and electron microscopy to investigate the dynamics among cell wall, cell membrane, and protoplasm during cell regeneration. Protoplasts were artificially induced in three species by cutting thalli and extruding the protoplasm. Protoplasts contracted and formed irregularly shaped masses within 30 minutes concomitant with bundling of actin filaments (AFs), convolution of cortical microtubules (CMTs) and formation of a thin enveloping membrane composed of polysaccharides.Size affected survival rates: protoplasts less than 10 μm in diameter displayed lower viabilitythan larger protoplasts. A new cell wall was produced within 24 hours simultaneous with CMT andAF depolymerization. AFs were reduced to granular structures and aggregates that repolymerizedby 48 hours. Concurrently, new CMTs polymerized and attained a parallel arrangement. Actin- andmicrotubule-destabilizing agents had variable effects on protoplast contraction indicating a minor role of intact cytoskeletons in this process; however, resulting cells exhibited abnormal protoplasm distribution and cell deformation after three days. Rhizoids began to form after 7 days on untreated cells which subsequently produced lateral branch cells that eventually developed into mature thalli.","subitem_description_type":"Abstract"}]},"item_4_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Graduate School of Kuroshio Science, Kochi University","subitem_publisher_language":"en"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1882823X","subitem_source_identifier_type":"ISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12321175","subitem_source_identifier_type":"NCID"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Paul, Rommel  Elvira"}],"nameIdentifiers":[{"nameIdentifier":"24414","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kazuo, Okuda"}],"nameIdentifiers":[{"nameIdentifier":"24415","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-23"}],"displaytype":"detail","filename":"kuro5-1.39.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"kuro5-1.39.pdf","url":"https://kochi.repo.nii.ac.jp/record/6454/files/kuro5-1.39.pdf"},"version_id":"de042f84-9d5f-4d0a-ab5a-e631206b2246"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"actin filament","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cortical microtubule","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protoplast","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Valonia","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cytoskeletal and Ultrastructural Studies on Regenerating Cells from the Protoplasts of Valonia (Siphonocladales, Chlorophyceae)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cytoskeletal and Ultrastructural Studies on Regenerating Cells from the Protoplasts of Valonia (Siphonocladales, Chlorophyceae)","subitem_title_language":"en"}]},"item_type_id":"4","owner":"3","path":["581"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-02-28"},"publish_date":"2012-02-28","publish_status":"0","recid":"6454","relation_version_is_last":true,"title":["Cytoskeletal and Ultrastructural Studies on Regenerating Cells from the Protoplasts of Valonia (Siphonocladales, Chlorophyceae)"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-21T00:48:45.039963+00:00"}