@article{oai:kochi.repo.nii.ac.jp:00002061,
 author = {Huang, Wei and Fujita, Yuji and Ninomiya, Masayori and Ohno, Masao},
 journal = {Bulletin of marine sciences and fisheries, Kochi University},
 month = {Mar},
 note = {application/pdf, Application of tissue culture techniques to seed production and cultivation of the red alga Grateloupia turuturu Yamada was examined. Callus masses excised from explants of G. turuturu exhibited an undifferentied growth on solid ASP12NTA (1.2% agar) medium supplemented with 0.1 mg/L of indole-3-acetic acid (IAA) and 6-benzylaminopurine (BAP). Growth of the callus was maintained for over five years by subculture every 2-3 months. The callus cells were able to attach to twines of Nori-net and oyster shell, following which they developed into crusts in PES liquid medium. The seeds were observed from upright development of the crusts.The young thalli grew to 3-5 mm in length after 6-8 weeks in room culture. For cultivation in sea, the Nori-nets were suspended from experimental rafts at Nagasaki and Kochi coast. The thalli grew most rapidly from January to April. After five months growth, the thalli attained a length of approxirnately which is similar to naturally growing thalli.},
 pages = {1--7},
 title = {Seed production and cultivation of Grateloupia turuturu (Cryptonemiales, Rhodophyta) by callus culture},
 volume = {19},
 year = {2000}
}