@article{oai:kochi.repo.nii.ac.jp:00002050,
 author = {川合, 研児 and 青山, 雅人},
 journal = {Bulletin of marine sciences and fisheries, Kochi University},
 month = {Mar},
 note = {application/pdf, Efficacy of the vaccine against Edwardsiella tarda infection in Japanese flounder Paralichthys olivaceus was studied by comparing the immunizing bacterial strains of different origins and three administration methods of the vaccine. Two groups of flounder, the mean body weight was about 53 g, were immunized intraperitoneally (IP) with formalin killed cells (FKCs) of E. tarda HH-1 strain and EF-1 strain. The serotypes of the two strain were preliminary clarified identical. Strain HH-1 was originally isolated from a flounder, and strain EF-1 was isolated from an eel. The immunized fish and unimmunized control fish were challenged by IP injection with strain HH-1. Comparison of different administration methods was executed using fish groups (about 53 g) immunized by IP injection, oral administration and immersion with FKC of strain HH-1 and challenged by IP injection with live cells of strain HH-1. Immune response (antibody titer in the serum and phagocytic activity) was also compared between groups of different immunization methods. Additionally, the effect of encapsulation of oral administration vaccine to examine anti-acid resistance of the vaccine in the stomach was examined by immunizing fish of 86 g with FKC of strain HH-1 then challenging with live cells of the same strain. The result in the mortality in the fish immunized with strains HH-1 and EF-1 was not significantly different at 5% level, which indicates origins of the strains does not affect efficacy of the vaccine as far as the serotype is sarne. Both survival rate and immune response were the highest in the group of IP injection followed by oral administration and immersion. Encapsulation of the vaccine in oral administration did not show advantageous effect than the uncapsulated vaccine. It may be attributed that the capsule did not release the vaccine antigen in the gastro-intestinal tract effectively.},
 pages = {35--43},
 title = {ヒラメのエドワジュラ症に対するワクチンの効果},
 volume = {20},
 year = {2001}
}