| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2026-03-20 |
| タイトル |
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|
タイトル |
Atractylodes lancea (Thunb.) DC. [Asteraceae] rhizome-derived exosome-like nanoparticles suppress lipopolysaccharide-induced inflammation in murine microglial cells. |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Atractylodes lancea (Thunb.) DC. [Asteraceae] rhizome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microglia |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
exosome-like nanoparticle |
| キーワード |
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|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Kampo medicine |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
microRNA |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
neuroinflammation |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 著者 |
Jobu Kohei
Kawada Kei
Isida Tomoaki
Morisawa Shumpei
Higashi Youichirou
Aizawa Fuka
Yagi Kenta
Izawa－Ishizawa Yuki
Niimura Takahiro
Abe Shinji
Goda Mitsuhiro
Miyamura Mitsuhiko
Ishizawa Keisuke
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| 抄録 |
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内容記述タイプ |
Other |
|
内容記述 |
Background: Exosome-like nanoparticles (ELNs) mediate interspecies intercellular communications and modulate gene expression. Hypothesis/Purpose: In this study, we isolated and purified ELNs from the dried rhizome of Atractylodes lancea (Thunb.) DC. [Asteraceae] (ALR-ELNs), a traditional natural medicine, and investigated their potential as neuroinflammatory therapeutic agents. Methods: ALR-ELN samples were isolated and purified using differential centrifugation, and their physical features and microRNA contents were analyzed through transmission electron microscopy and RNA sequencing, respectively. BV-2 microglial murine cells and primary mouse microglial cells were cultured in vitro, and their ability to uptake ALR-ELNs was explored using fluorescence microscopy. The capacity of ALR-ELNs to modulate the anti-inflammatory responses of these cells to lipopolysaccharide (LPS) exposure was assessed through mRNA and protein expression analyses. Results: Overall, BV-2 cells were found to internalize ALR-ELNs, which comprised three microRNAs (ath-miR166f, ath-miR162a-5p, and ath-miR162b-5p) that could have anti-inflammatory activity. Pretreatment of BV-2 cells with ALR-ELN prevented the pro-inflammatory effects of LPS stimulation by significantly reducing the levels of nitric oxide, interleukin-1β, interleukin-6, and tumor necrosis factor-α. Notably, the mRNA levels of Il1b, Il6, iNos, ccl2, and cxcl10 in BV-2 cells, which increased upon LPS exposure, were significantly reduced following ALR-ELN treatment. Moreover, the mRNA levels of heme oxygenase 1, Irf7, ccl12, and Irg1 also increased significantly following ALR-ELN treatment. In addition, pretreatment of primary mouse microglial cells with ALR-ELN prevented the pro-inflammatory effects of LPS stimulation by significantly reducing the levels of nitric oxide. Conclusion: Our findings indicate that ALR-ELNs exhibit anti-inflammatory effects on murine microglial cells. Further validation may prove ALR-ELNs as a promising neuroinflammatory therapeutic agent. |
|
言語 |
en |
| 書誌情報 |
en : Frontiers in pharmacology
巻 15,
号 1,
p. 1302055,
発行日 2024-04-26
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| ISSN |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
1663-9812 |
| DOI |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.3389/fphar.2024.1302055 |
| 権利 |
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|
権利情報 |
© 2024 Kawada, Ishida, Morisawa, Jobu, Higashi, Aizawa, Yagi, Izawa-Ishizawa, Niimura, Abe, Goda, Miyamura and Ishizawa. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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言語 |
en |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
Frontiers Media S.A. |
|
言語 |
en |
fphar-15-1302055.pdf (1.7 MB)
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