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Production of Monoclonal Antibodies against Yellowtail Leucocyte Ishikawa, Takahide Kawai, Kenji Monoclonal antibody yellowtail leucocyte classification of leucocytes application/pdf BALB/c mouse was immunized with yellowtail peripheral blood leucocytes (PBLs) and spleen cells removed from the immunized mouse were fused with myeloma by using polyethyleneglycol method. Antibody producing hybridomas were screened by ELISA using mixed leucocytes as target antigen. A total of 8 hybridomas (culture no. AB-2, AB-10, BB-11, BC-5, BC-6, CF-11, DG-10 and EG-11) were obtained by single cell cloning. Monoclonal antibodies (MAbs) produced in the culture supernatant of cloned hybridomas were used for further characterization. Imunofluorescent stain using FITC labeled rabbit secondary antibody revealed that MAbs AB-2, AB-10 and EG-11 recognized lymphocyte and MAbs BC-5, CF-11 and DG-10 recognized granulocyte or monocyte. MAbs BC-6 and BB-11 reacted to yellowtail lymphocyte which bore immunoglobulin of yellowtail. 高知大学海洋生物教育研究センター 1998-12-31 eng departmental bulletin paper VoR http://hdl.handle.net/10126/4351 https://kochi.repo.nii.ac.jp/records/2066 03879763 AA10778872 Bulletin of marine sciences and fisheries, Kochi University 18 27 31 https://kochi.repo.nii.ac.jp/record/2066/files/marine18-027.pdf application/pdf 1.0 MB 2018-04-23